Investigation of the Attachment of Circulating Endothelial Cells to a Cell Probe: Combined Experimental and Numerical Study

Circulating endothelial cells (CECs) are a reliable biomarker for cardiovascular diseases (CVDs). A major unresolved challenge limiting the widespread use of CECs diagnosis and monitoring CVDs is their unreliable detection. This problem mainly attributed to low sample volume (5–10 mL) commonly used ex vivo CEC isolation methods. To overcome this limitation, BMProbe in proposed. It consists twisted medical flat wire with polymer-coated surface functionalized anti-CD105 antibodies. combined experimental numerical study performed investigate which flow conditions lead an increased cell attachment probe's surface. Endothelial solved dextran solution circulated system containing BMProbes. Microscopic images attached taken. In addition, experiments simulated using computational fluid dynamics (CFD) solver quantify at The microscopic superimposed CFD data influence wall shear rate normal on probe. Most all (85.5%) found areas negative rate.